Additional file 3.

Supplementary Figure S3. Complementation analysis of Ulp1-GFP fusion constructs. ULP1-GFP and ULP1(3)-GFP fusion constructs complement the growth phenotype of a ulp1 deletion strain. A ulp1 shuffle strain (ulp1::HIS3, ULP1/URA3) was transformed with one of the following low-copy plasmids: ULP1-GFP, ULP1-region 1-GFP, ULP1-region 2-GFP, ULP1(3)-GFP, ULP1(3)C580S-GFP or the empty vector. Transformants were streaked onto yeast extract peptone dextrose medium and then medium containing 5-fluoroorotic acid to select for loss of the ULP1/URA3 plasmid. Note that, as expected, only the ULP1-GFP and ULP1(3)-GFP fusion constructs complement the growth phenotype of the ulp1 deletion strain.

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Elmore et al. BMC Biology 2011 9:74   doi:10.1186/1741-7007-9-74