Additional file 3.
Doxorubicin-induced autophagy in Cas-deficient cells. Cas -/- (EV) and Cas-FL cells were treated or not with 2 μM Doxorubicin for 24 hours, and the expression of p62 and LC3-II was examined by immunoblot. Relative intensities of p62 and LC3-II were calculated by normalizing the untreated control in Cas -/- (EV) cells to a relative intensity of 1.0, and then dividing subsequent p62 or LC3-II bands intensity by actin bands intensity in the same treatment group using densitometry.
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Zhao and Vuori BMC Biology 2011 9:73 doi:10.1186/1741-7007-9-73