Additional file 2.

MG132-induced autophagy in Cas-deficient cells as measured by p62 levels. (A) Cas -/- (EV) and Cas-FL cells were treated or not with 1 μM MG132 for 16 hours, and p62 expression was examined by immunoblot. Relative intensity of p62 was calculated by normalizing the untreated control in Cas -/- (EV) cells to a relative intensity of 1.0, and then dividing subsequent p62 band intensity by actin band intensity in the same treatment group using densitometry. (B) HeLa cells transiently transfected with Cas shRNAs or control shRNA were treated with 2.5 μM MG132 for 24 hours, and then assayed for p62 levels. The results (normalized to the untreated control) are presented as in panel A.

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Zhao and Vuori BMC Biology 2011 9:73   doi:10.1186/1741-7007-9-73