Additional file 2.
Identification of a putative 5-fluorouracil transporter by the robot-assisted experiment. (a) Control plate with homozygous deletion mutant strains spotted in quadruplicates (by a Singer RoToR© HAD robot) onto F1 agar medium containing 1% DMSO. (b) Identity of deletion mutants spotted onto control and drug plates. (c). Homozygous deletion mutant strains spotted in quadruplicates (by a Singer RoToR© HAD robot) onto F1 medium agar plate containing 158 μM 5-fluorouracil. (d) Quantification of the relative growth between drug and control plates with 5-fluorouracil resistant strains highlighted in green.
Format: PPT Size: 552KB Download file
This file can be viewed with: Microsoft PowerPoint Viewer
Lanthaler et al. BMC Biology 2011 9:70 doi:10.1186/1741-7007-9-70