Additional file 9.

Mutations of the crystal dimer interface do not prevent oligomerisation. (a) Oligomerisation of 80 μM WT or mutant Irga6 proteins was monitored by light scattering in the presence of 10 mM GTP at 37°C. (b) Hydrolysis of 10 mM GTP (with traces α32P-GTP) was measured in the presence of 80 μM WT or mutant Irga6 proteins at 37°C. Samples were assayed by TLC and autoradiography.

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Pawlowski et al. BMC Biology 2011 9:7   doi:10.1186/1741-7007-9-7