Figure 2.

Localization of axon initial segments (AISs) in motor neurons (MNs). Triple immunostaining of Peripherin (A, F), ankyrin G (AnkG) (B, G) (brackets indicate the AIS) and microtubule-associated protein 2 (MAP2) (C, H) (AnkG and MAP2 are merged in (D, I)) in the adult mouse lumbar spinal cord showing two populations of MN AISs: emanating directly from the soma (A-E) or from a primary MAP2⁺ dendrite (F-J). (E, J) Simplified drawing of the merged image, respectively in (D) and (I). Note that the green MAP2 staining progressively decreases within the red AnkG⁺ AIS. Scale bar = 10 μm.