Additional file 1.
Quantitative RT-PCR comparing the transcript levels for dm, cyclin D and cyclin E in Drosophila S2 cells upon insulin (A) or amino acids (B) treatment. Cells were treated with insulin or AAs and RNA was extracted at the indicated times. qRT-PCRs were performed to analyze expression of diminutive (dm), cyclin D and cyclin E RNAs. The sequences of the primers used are available in Additional file 8; Supplementary Material and Methods. actin was used as the internal control. Error bars indicate the standard deviations (±) calculated on the average of three separate experiments.
Format: JPEG Size: 465KB Download file
Parisi et al. BMC Biology 2011 9:65 doi:10.1186/1741-7007-9-65