Additional file 1.

Comparison of HES5 protein and promoter sequences. (A, B) Analysis of sequence similarity between the different HES5 proteins (A), and the corresponding phylogenetic tree (B), shows that among all three chick HES5 proteins, HES5-1 has the highest degree of homology with mammalian HES5. (C) Sequence alignment of the proximal 400 bp promoter regions of several Hes5-like genes. Shaded areas represent regions of homology and highlight several conserved regions, such as TATA box, CCAAT box and CSL binding sites. The two identified high-affinity CSL-binding sites have been previously reported to be essential for Notch-mediated promoter activity [15,22,26,54]. The Hes5-1 promoter has the highest homology to mammalian Hes5 promoters and was included in the pHes5-VNP reporter.

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Vilas-Boas et al. BMC Biology 2011 9:58   doi:10.1186/1741-7007-9-58