Monitoring of Notch activity in embryos electroporated with pHes5-VNP and pCAG-CherryNLS. (A, B, C) Neural tube slices of embryos transfected with pHes5-VNP and pCAG-CherryNLS were imaged in a wide-field microscope. The selected images in (A) show that Notch activity is detected before and during mitosis, with the two daughter cells containing similar levels of Notch activity. In (B), Notch activity is detected after mitosis in only one of the two daughter cells, while in (C) Notch becomes active after mitosis in both daughter cells, but at different times. Images presented are from maximum intensity projections. (A', B', C') Charts showing the pHes5-VNP fluorescence intensities of the (A, B, C) cell lineages along time, with mitosis as a reference point. Arrows in (A', B', C') indicate the onset of Notch activity.
Vilas-Boas et al. BMC Biology 2011 9:58 doi:10.1186/1741-7007-9-58