Resolution:
## Figure 7.
Quantification of PMM depletion phenotypes in HeLa cells. Images from HeLa cells as described in Figure 6 were quantified for phenotypical
changes. ( Cell-cell adhesion. A junction was counted as adherent if more 50% of the length
of that cell side was in contact with the neighbouring. The number of sides with junctions
was divided by the total number of sides to give the percentage of adherent junctions.
The number of sides varied between three to eight/cell. In each experiment 40 cells
in each of two images were quantified per condition; therefore, total cell number
analysed/condition = 120. A)(B) Microspikes. In each experiment, protrusions from the cell edge were counted for
16 cells in each of two images; therefore, the total number of cell edges analysed/condition
= 96. ( Nuclear morphology. In each experiment, three fields of cells (50 to 200 cells/field)
were counted per condition. Only interphase cells were counted. Misshapen nuclei were
defined as those deviating substantially from an oval shape. Graphs show the mean
± SD of two independent experiments.
C)Bai |