Additional file 1.

Histograms of mCherry-green fluorescent protein (GFP) (tandem)-microtubule-associated protein 1 light chain 3 B (LC3) and GFP-Rab7 measurements. Tandem-LC3 (a,b) or GFP-Rab7 (c) cells were exposed to full medium (FM), nutrient deprivation (ND) or FM + bafilomycin A1 (Baf) conditions and fluorescence intensities were analyzed by flow cytometry. Histograms represent distribution of fluorescence intensities of GFP (a) or mCherry (b) of tandem-LC3 and GFP-Rab7 (c) after 6 h (upper rows) or 16 h (lower rows) incubation with FM, ND or FM + Baf.

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Hundeshagen et al. BMC Biology 2011 9:38   doi:10.1186/1741-7007-9-38