Figure 6.

Confirmation of autophagic flux induction by cardiac glycosides. (a) Representative images of mCherry-green fluorescent protein (GFP)-microtubule-associated protein 1 light chain 3 B (LC3) cells; exposed to full medium (FM), nutrient deprivation (ND), FM + digoxin, strophanthidin or digoxigenin (each at 100 ng/ml) in presence/absence of bafilomycin A1 (Baf) for 6 h. (c) Wild-type cells were incubated with FM, ND, FM + digoxin, strophanthidin or digoxigenin (each at 100 ng/ml) in presence/absence of Baf for 6 h. Cell lysates were analyzed for LC3 and β-actin levels by western blotting.

Hundeshagen et al. BMC Biology 2011 9:38   doi:10.1186/1741-7007-9-38
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