Table 1

Quantification of 13C enrichments in relevant metabolites by 1H total correlation spectroscopy (TOCSY) at 48 h

Compound

Percentage 13C


Uracil in UXP


12C612C5

52 ± 3


12C613C5

14 ± 2


13C612C5

16 ± 2


13C613C5

18 ± 2


Asp


12C212C3

46 ± 3


12C213C3

15 ± 2


13C212C3

19 ± 2


13C213C3

21 ± 2


AXP ribose


12C1'12C2'

8 ± 2


13C1'13C2'

92 ± 2


UXP ribose


12C1'12C2'

4 ± 1


13C1'13C2'

96 ± 2


UDP GlcNAc


Glc 12C112C2

9 ± 2


Glc 13C113C2

91 ± 2


Lactate


12C212C3

19 ± 2


13C213C3

81 ± 2


Alanine


12C212C3

23 ± 2


13C213C3

77 ± 2


Glutamate


12C212C4

63 ± 2


12C213C4

32 ± 2


13C212C4

< 1


13C213C4

5 ± 2


12C212C3

81 ± 2


12C213C3

6 ± 2


13C212C3

5 ± 2


13C213C3

8 ± 2


Atom percentage 13C enrichments in metabolites after 48 h incubation in [U-13C]-glucose were determined from two-dimensional 1H TOCSY experiments as described in the Methods section. Uracil in UXP refers to the uracil ring in the free nucleotide pool comprising UTP, UDP and UMP. AXP and UXP ribose refer to the ribose moiety in the free nucleotide pools. UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) refers to the C1-C2 of the glucose moiety of the free UDP-GlcNAc pool.

Moseley et al. BMC Biology 2011 9:37   doi:10.1186/1741-7007-9-37

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