Resolution:
## Figure 7.
Deconvolution of isotopologues of UDP-. Mass isotopologues at each timepoint were deconvoluted into individual isotopomer
components and their intensity was corrected for natural abundance contribution, as
described in the Methods section. N-acetyl-D-glucosamine (UDP-GlcNAc) and kinetic modeling of their timecourses(a) Mole fractions of the various components are plotted with time. m_{0 }(open square), ^{13}C_{6}-glucose g6 (black filled square),^{13}C_{5}-ribose r5 (open circle), ^{13}C_{2}-acetyl a2 (black filled circle), ^{13}C_{1}-uracil u1 (blue filled square),^{13}C_{2}-uracil u2 (green filled square), ^{13}C_{3}-uracil u3 (red filled square). The ribose component was fitted to the function b(1-exp-kt)
with b = 0.89 and k = 0.13 h^{-1 }R^{2 }= 0.977. The unlabeled species (m_{0}) was fitted to a single exponential decay I(t) = I(0)exp(-kt) with I(0) = 0.88 ±
0.06, k = 0.13 ± 0.02 h^{-1 }R^{2 }= 0.967. (b) Reconstruction of the isotopologue distribution from the mole fraction probabilities
in A for the 48 h timepoint. The best fit values for the fractions were obtained according
to equations 3,4 and 5 using the 'Genetic Algorithm for Isotopologues in Metabolic
Systems' (GAIMS) as described in the text. The symbols are given on the figure. Blue
bars are the observed intensities at each m/z and red bars are the reconstructed intensities.
Moseley |