Mutant rpn1 alleles display genetic interactions with mutations in genes for intrinsic ubiquitin receptors. Five-fold serial dilutions of cells were plated onto the indicated media. The rpn1 mutants (rpn1*) were plasmid shuffled into an rpn1Δ strain containing (from top to bottom) either no additional mutations (A) or rpn13Δ (B), rpn13-KKD (C), rpn10- uim (D), rpn13-KKD rpn10-uim (E), or rpn4Δ (F). AZC refers to 5 mM of the proline analog l-azetidine-2- carboxylic acid (AZC)"
Gomez et al. BMC Biology 2011 9:33 doi:10.1186/1741-7007-9-33