Additional File 7.

Effect of mepyramine on Gq fluorescence resonance energy transfer (FRET) ratio changes in the presence of the guanine nucleotide exchange factor p63RhoGEF Representative trace depicting the FRET ratio change (yellow fluorescent protein:monomeric Turquoise (YFP:mTq)) upon addition of histamine (100 μmol/l) in HeLa cells coexpressing Gαq-mTqΔ6, Gβ1, YFP-Gγ2 and p63RhoGEF. Addition of the histamine 1 receptor (H1R) inverse agonist mepyramine (10 μmol/l) reversed the ratio change induced by histamine. (Inset) the mTq and YFP intensity traces from which the ratio was derived.

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Adjobo-Hermans et al. BMC Biology 2011 9:32   doi:10.1186/1741-7007-9-32