Fluorescence lifetime data. Average cyan fluorescent protein (CFP) lifetimes measured under different conditions. The phase lifetime of Gαq-mTqΔ6 was measured in the absence (n = 15) and presence (n = 12) of the acceptor YFP-Gγ2. The difference in lifetime was significant. The Gq sensor was coexpressed with the histamine H1 receptor (H1R) and CFP lifetimes were measured before and after addition of 100 μmol/l histamine (n = 6). The Gq sensor was coexpressed with the guanine nucleotide exchange factor p63RhoGEF and CFP lifetimes were measured before and after addition of 100 μmol/l histamine (n = 6). In both conditions the lifetime difference before and after stimulation was significant. The CFP lifetimes before addition of histamine are not different (Student t-test, 95% CI) in the presence or absence of either H1R or p63RhoGEF.
Adjobo-Hermans et al. BMC Biology 2011 9:32 doi:10.1186/1741-7007-9-32