Gαq-visible fluorescent protein (VFP) is functional. (A) HeLa cells expressing Gαq tagged with monomeric yellow fluorescent protein (Gαq-mYFP). (B) Mouse embryonic fibroblasts (MEFs) derived from wild-type mice showed an increase in [Ca2+]i upon addition (arrow) of bradykinin (BK) (1 μmol/l, n = 6. Error bars depict SE). (C) MEFs derived from Gαq/11-deficient mice (MEFq/11-/-) did not display increased [Ca2+]i upon addition of BK (n = 9). (D) Expression of wild-type Gαq in MEFq/11-/- caused an increase in cytosolic calcium upon addition of BK (n = 2). Most cells did not express the re-introduced wild-type (untagged) Gαq and did not show a decrease in Fura Red intensity upon addition of BK. (E) Expression of Gαq-mYFP in MEFq/11-/- caused an increase in cytosolic calcium upon addition of BK (n = 12); (Inset) MEFq/11-/- cell expressing Gαq-mYFP. (F) Gαq-mTqΔ6 similarly caused an increase in [Ca2+]i upon addition of BK (n = 6). (Inset) MEFq/11-/- cell expressing Gαq-mTqΔ6.
Adjobo-Hermans et al. BMC Biology 2011 9:32 doi:10.1186/1741-7007-9-32