Phosphorylation of DNMT1 by PKCζ reduces its methyltransferase activity. Quantitative measurements of S-adenosyl-l-(methyl-3H)methionine integration in a DNA matrix poly(dI-dC).poly(dI-dC) by 20 nM of recombinant DNMT1 in the presence 100 ng of recombinant PKCζ incubated with or without 50 μM of ATP for different times. Data are representative of three independent experiments. Bars, S.D.
Lavoie et al. BMC Biology 2011 9:31 doi:10.1186/1741-7007-9-31