Expression of constitutively active erythropoietin receptor in pyramidal neurons of cortex and hippocampus boosts higher cognitive functions in mice
- Equal contributors
1 Division of Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Hermann-Rein-Str.3, 37075 Göttingen, Germany
2 DFG Research Center for Molecular Physiology of the Brain (CMPB), Humboldtallee 23, 37073 Göttingen, Germany
3 Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Hermann-Rein-Str.3, 37075 Göttingen, Germany
4 Department of Neuro- and Sensory Physiology, Georg-August-University, Humboldtallee 23, 37073 Göttingen, Germany
5 Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, Hermann-Rein-Str.3, 37075 Göttingen, Germany
BMC Biology 2011, 9:27 doi:10.1186/1741-7007-9-27Published: 28 April 2011
Additional file 1:
Expression of HA-cEPOR in the 'TG2' line. Forebrain-specific expression of HA-cEPOR transgene also in line 'TG2' was revealed by immunohistochemistry. A monoclonal antibody against the HA-tag was used to stain coronal sections of hippocampus. HA-cEPOR expression is absent in WT mice (A and B). Similar to the line 'TG1', HA-cEPOR expression is restricted to the pyramidal neurons of cortex (C), CA1 (D), CA3 subregions of hippocampus and granular layer of dentate gyrus. Scale bars; 100 μm and 500 μm.
Format: JPEG Size: 933KB Download file
Additional file 2:
Overexpression of cEPOR does not affect overall brain morphology. (A) Coronal sections from WT and TG mice were stained with haematoxylin-eosin and (B) Luxol Fast Blue. General brain morphology and myelin architecture were comparable between WT and TG mice. Scale bars; 1 mm.
Format: JPEG Size: 1MB Download file
Additional file 3:
Within-day trial analysis in the hidden platform paradigm of Morris water maze. In cEPOR TG mice, there is a tendency of faster within-day learning, that is shorter latency to reach the platform on days one and two. However, on most of the training days, ANOVA did not reach significance levels. WT n = 18; TG n = 13; mean ± SEM presented.
Format: PDF Size: 17KB Download file
This file can be viewed with: Adobe Acrobat Reader