Additional file 5.

Figure S4. Purification of recombinant isomin. (A) Purified inclusion bodies contain a band of about 66 K, corresponding to the fusion protein GST-isomin. (B) After treatment of inclusion bodies with the preScission protease, isomin still occurs in the insoluble fraction (P), while GST is solubilised (S). (C) Urea treatment results in the solubilization of about 50% isomin from inclusion bodies. Electrophoresis on a 12% SDS-polyacrylamide gel.

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Mencarelli et al. BMC Biology 2011 9:17   doi:10.1186/1741-7007-9-17