Subcellular localization of native Evc and Evc2. Each row consists of images from one representative cell. (a). Evc (S43B; red) and Evc2 (R1656; green) colocalize at the base of the primary cilium in an IMCD3 cell. γ- and acetylated tubulin antibodies (both blue) identify the centrioles and the primary cilium respectively. Evc2 has an additional pericentriolar distribution. Scale bar 5 μm. (b). Evc is located along the length of the ciliary axoneme in MC3T3 cells. Evc (S43B; red) colocalizes with the primary cilia marker acetylated tubulin (green). Scale bar 10 μm. (c). Evc2 is concentrated at the base of the primary cilium and only partially colocalizes with Evc in MC3T3 cells. Evc (S43B; red) and Evc2 (R1656; green). Scale bar 10 μm.
Blair et al. BMC Biology 2011 9:14 doi:10.1186/1741-7007-9-14