Figure 1.

qPCR of a pluripotent cell type compared with cells of the regeneration blastema. A) Relative expression of pluripotency associated genes in Xenopus cap cells compared to limb blastema (st52) at different time points during regeneration. In red is relative expression of animal cap cells, in green the relative expression of st52 limb blastemas at 0 dpa, 1 dpa, 3 dpa and 5 dpa. (note: the results for oct25, oct60 and oct79 expression was combined as oct4 expression). B) Relative expression of pluripotency associated genes in Xenopus cap cells and st52 tail blastema cells. C) Relative expression of pluripotency associated genes in Xenopus cap cells and regenerative incompetent pseudo blastemas of st57 limbs at 0 dpa, 1 dpa, 3 dpa, and 5 dpa. Asterisks indicate significant (P < 0.05) upregulation of expression compared to 0 dpa. D) Relative gene expression for pluripotency associated markers in zebrafish non-regenerating (0 hpa), regenerating (6 hpa to 72 hpa) fin and embryonic cells in oblong stage. E) Relative expression of blastema markers in cap cells and regenerating limbs. Asterisks indicate significant (P < 0.05) upregulation of gene expression compared to the previous time point. F) Relative expression of blastema markers in zebrafish non-regenerating (0 hpa), regenerating (6 hpa to 72 hpa) fin and embryonic cells in oblong stage. For each gene, expression was normalized to highest expressing tissue. Error bars indicate Standard deviation (SD). (For statistical differences see Additional file 1).

Christen et al. BMC Biology 2010 8:5   doi:10.1186/1741-7007-8-5
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