Open Access Research article

The zebra finch neuropeptidome: prediction, detection and expression

Fang Xie1, Sarah E London26, Bruce R Southey13, Suresh P Annangudi16, Andinet Amare1, Sandra L Rodriguez-Zas235, David F Clayton2456 and Jonathan V Sweedler1256*

Author Affiliations

1 Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 USA

2 Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 USA

3 Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 USA

4 Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 USA

5 Neuroscience Program, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 USA

6 Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL, 61801 USA

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BMC Biology 2010, 8:28  doi:10.1186/1741-7007-8-28

Published: 1 April 2010

Additional files

Additional file 1:

The sequences and masses of identified peptides in the zebra finch by mass spectrometry. Peptides were identified by searching the mass spectrometry (MS) data against the database of predicted prohormones in the zebra finch. Except those three marked with an asterisk (*) that were identified by mass match, all of the others were confirmed by MS/MS sequencing. Each individually detected and sequenced peptide is listed. Common posttranslational modifications were also characterized for some peptides. p- (N-terminal pyroglutamate formation); -amide (C-terminal amidation); Ac- (acetylation); C (disulfide bond). : annotated as neuropeptide based on the chicken prohormone gene database.

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Open Data

Additional file 2:

Zebra finch prohormone and signaling sequences. Predicted sequences of zebra finch prohormone and signaling genes in FASTA format.

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Open Data