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Open Access Research article

In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA

Maria Laine P Tinoco123, Bárbara BA Dias1, Rebeca C Dall'Astta13, João A Pamphile4 and Francisco JL Aragão123*

Author Affiliations

1 Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, 70770-900, Brasília, DF, Brazil

2 Universidade de Brasília, Departamento de Biologia Celular, Campus Universitário, 70910-900, Brasília, DF, Brazil

3 Instituto Nacional de Ciência e Tecnologia em Interações Planta-Praga, Universidade Federal de Viçosa, 36570.000, Viçosa, MG, Brazil

4 Universidade Estadual de Maringá, Departamento de Genética e Biologia Celular, Maringá, PR, Brazil

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BMC Biology 2010, 8:27  doi:10.1186/1741-7007-8-27

Published: 31 March 2010

Abstract

Background

Self-complementary RNA transcripts form a double-stranded RNA (dsRNA) that triggers a sequence-specific mRNA degradation, in a process known as RNA interference (RNAi), leading to gene silencing. In vascular plants, RNAi molecules trafficking occur between cells and systemically throughout the plant. RNAi signals can spread systemically throughout a plant, even across graft junctions from transgenic to non-transgenic stocks. There is also a great interest in applying RNAi to pathogenic fungi. Specific inhibition of gene expression by RNAi has been shown to be suitable for a multitude of phytopathogenic filamentous fungi. However, double-stranded (ds)RNA/small interfering (si)RNA silencing effect has not been observed in vivo.

Results

This study demonstrates for the first time the in vivo interference phenomenon in the pathogenic fungus Fusarium verticillioides, in which expression of an individual fungal transgene was specifically abolished by inoculating mycelial cells in transgenic tobacco plants engineered to express siRNAs from a dsRNA corresponding to the particular transgene.

Conclusion

The results provide a powerful tool for further studies on molecular plant-microbe and symbiotic interactions. From a biotechnological perspective, silencing of fungal genes by generating siRNAs in the host provides a novel strategy for the development of broad fungi-resistance strategies in plants and other organisms.