Additional file 2.
Figure S2. Data supporting the time stability of RNA profiles. (A) Profiles of Actin11 transcript density. Blue and red symbols correspond to the data shown in Figure 2A. Data were split into two groups according to their sampling time. The black lines correspond to complementary data - three independent roots collected after more than 72 hours of continuous dark. (B) Additional arguments for the steadiness of expression profile. The assumption of a steady profile for Actin11 is supported by the mining and viewing of diurnal and circadian microarray data from Arabidopsis and poplar http://diurnal.cgrb.oregonstate.edu webcite. Figure S2B shows the diurnal patterns of Actin expression in leaves, which are the most exposed to light and presumably the most light-sensitive organ. The Arabidopsis ortholog (At3g12110) (dashed lines) exhibited a diurnal regulation of its expression with no circadian persistency. On the contrary, poplar Actin11 (continuous lines) showed a very constant expression over the diurnal cycle and no circadian rhythm. The absence of circadian control in poplar leaf supports the hypothesis of constancy of Actin11 expression in roots. Blue and red = 12 h light/12 h dark; black = continuous light; dashed black = continuous dark. Temperature was constant (22°C).
Format: PPT Size: 71KB Download file
This file can be viewed with: Microsoft PowerPoint Viewer
Merret et al. BMC Biology 2010 8:18 doi:10.1186/1741-7007-8-18