Additional file 2.
Movie 2 - Different modes of interaction with budded yeast. The cell expresses PHcrac-GFP (green), a label for phosphoinositides PI(3,4,5)P3 and PI(3,4)P2 and mRFP-LimEΔ (red) for actin filaments. The fluorescence images are superimposed on greyscale brightfield images. This sequence, corresponding to Figure 2a shows cup extension, retraction to the neck, and severing of the particle. Frame to frame interval is 4 seconds. All Additional files are movies showing phagocytes of Dictyostelium discoideum that interact with living yeast particles. Bars in the movies indicate 10 μm.
Format: MOV Size: 624KB Download file
Clarke et al. BMC Biology 2010 8:154 doi:10.1186/1741-7007-8-154