Table 3

Quantification of p27 and p21 protein levels in protein prostate cancer cell lines, HeLa and IMR90 cells treated with 40 μM small molecule inhibitors of p27 depletion (SMIPs) for 24 h.

LNCaP-S14

LNCaP

PC3

DU145

HeLa

IMR90

SMIP

p27

p21

p27

p21

p27

p21

p27

p21

p27

p21

p27

p21


DMSO

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

1.0 ± 0.0

SMIP001

1.9 ± 0.5

2.0 ± 0.6

1.5 ± 0.7

1.7 ± 0.6

1.3 ± 0.6

1.1 ± 0.5

1.1 ± 0.2

1.1 ± 0.3

1.1 ± 0.1

1.1 ± 0.2

1.0 ± 0.0

1.1 ± 0.0

SMIP004

2.4 ± 0.7

2.8 ± 0.7

2.1 ± 1.1

2.7 ± 0.1

1.1 ± 0.3

1.7 ± 0.9

1.3 ± 0.1

1.7 ± 0.5

1.1 ± 0.2

1.1 ± 0.1

1.2 ± 0.3

1.1 ± 0.0

MG132

1.5

1.9 ± 0.1

2.9

2.0 ± 1.0

4.1 ± 0.4

2.4 ± 0.1

3.1

2.2 ± 0.9

5.9 ± 1.7

0.9 ± 0.5

1.1 ± 0. 1

Bortezomib

1.8

2.1

2.5 ± 1.0

4.7 ± 1.4

1.7 ± 0.5

2.5 ± 0.4

1.3 ± 0.2

2.2 ± 0.8

1.8 ± 0.0

1.8 ± 0.4


The data is representative of at least four independent immunoblotting experiments.

Rico-Bautista et al. BMC Biology 2010 8:153   doi:10.1186/1741-7007-8-153

Open Data