Additional file 8.

Supplementary Figure 6. The ChIn assay can be adapted to carry out an inflammation resolution screen. BACmpx::GFP transgenic fish were raised and treated at 56 hpf with 10 μM CuSO4 as described for the ChIn assay. The number of infiltrating leukocytes in the lateral line were counted and compared to those of untreated fish at 0 hours posttreatment (hpt). These fish were then left for a further 3 hours after removal of copper (3 hpt) and examined once again to count infiltrating leukocytes. Copper-treated fish show significant diminishment of leukocyte numbers by this time, indicating resolution of inflammation. If copper-treated fish are exposed to diclofenac or ibuprofen at 0 hpt and examined at 3 hpt, the number of infiltrating leukocytes remains high, indicating that these drugs inhibit resolution.

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d'Alençon et al. BMC Biology 2010 8:151   doi:10.1186/1741-7007-8-151