Additional file 2.


Supplementary Movie 1. Migration of leukocytes toward damaged neuromasts. Transgenic BACmpx::GFP larvae were stained with 3,3'-dihexyloxacarbocyanine iodide (DiOC6) to reveal neuromasts (red arrows; individual labeled cells on the skin are chemosensory cells). In the movie, the trunk and part of the tail are shown anterior to the left. The images are displayed without pseudocoloring, given that DiOC6 is visible in the green fluorescent protein (GFP) fluorescence channel. After adding 50 μM CuSO4 to the medium, fish were mounted and immediately imaged for 2 hours to observe the behavior of leukocytes. Two GFP-labeled cells have been colored red to follow their trajectories toward the nearest neuromast. A wavelike contraction of the neuromast can be seen near the beginning of the sequence. Note the large number of leukocytes that arrive at the posterior-most neuromast after copper damage. Original magnification, ×20.

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d'Alençon et al. BMC Biology 2010 8:151   doi:10.1186/1741-7007-8-151