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Open Access Research article

Spontaneous focal activation of invariant natural killer T (iNKT) cells in mouse liver and kidney

Jia Zeng12 and Jonathan C Howard1*

Author Affiliations

1 Institute for Genetics, University of Cologne, Zuelpicher Strasse 47a, 50674 Cologne, Germany

2 Section of Experimental Therapeutics, Leeds Institute of Molecular Medicine, Level 7, Wellcome Trust Brenner Building, St James's University Hospital, Leeds LS9 7TF, UK

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BMC Biology 2010, 8:142  doi:10.1186/1741-7007-8-142

Published: 30 November 2010

Additional files

Additional file 1:

Table S1 - Quantification of Irga6 focal expression patches in liver and kidneys of various mouse strains. Focal Irga6 expression was quantified on stained histological sections of liver and kidney from strains of mice listed, as described in Materials and Methods. Each value in the table is the mean number of expression foci per microscope field averaged over 30 fields. WT = wild type; KO = knock-out, IFN R are mice lacking IFN-type I (IFNAR) and type II (IFNGR) receptors respectively; SPF = specific pathogen free; GF = germ-free; Portugal, Sweden and Switzerland refer to the national origins of three independent germ-free C57BL/6 strains.

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Additional file 2:

Figure S1 - Irga6 is over-expressed in the kidney of RAG and JHT mice. Paraffin sections from kidneys of RAG or JHT mice were probed for Irga6 protein (Green). Nuclei were counterstained in red. Kidneys from WT control mice were also included.

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Additional file 3:

Figure S2 - Irgm3 is co-expressed at Irga6 expression foci in liver and kidney. Serial paraffin sections (6 μm) of organs from C57BL/6 adult mice were prepared. In each case, two adjacent serial sections were probed for Irga6 (1, green) and Irgm3 protein (2, green) respectively. Frames show enlarged images. Nuclei were counter-stained in red.

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Additional file 4:

Table S2 - List of primers used in RT-PCR and Real time PCR.

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Additional file 5:

Table S3 - Sequence comparison of identified TCR clones. Sequences of identified TCR Vβ8 (A) and Vα14/11 (B) clones from Irga6 liver cored patches, and sequences of identified TCR Vα11 (C) clones from lymph nodes were compared and classified into different junction types. The number of clones belonging to each junction type was listed. Vβ, Dβ, Jβ, Vα and Jα subfamily names were labeled (' - ' means not identifiable). TCR Vβ-N(D)N-Jβ or TCR Vα-N-J junctional sequences were displayed. 'Short' indicates that the length of the sequence was too short to identify the entire junctional region.

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