Additional file 2.
Figure S2 - Species-specific mechanisms for the generation of secreted carcinoembryonic antigen related cell adhesion molecules (CEACAMs). (A) Formation of secreted CEACAM proteins by truncation of A domains in the horse by nonsense mutations. Partial nucleotide sequence of A domain exons from putative secreted CEACAMs (composed of a leader, an N domain and an A domain) of the horse have been aligned. The sequences (truncation at the 3'-end is indicated by dots) are closely related except for CEACAM48. Stop codons are indicated in red. Homologous codons of CEACAM44, CEACAM46, CEACAM47 and CEACAM49 which could be changed into stop codons by one mutation are indicated in blue. Note that there is a common stop codon starting at nucleotide position 63 (TAA). Additional in-frame stop codons in CEACAM46 and CEACAM44 lead to a shortening of the A domain of the secreted molecule. (B) Formation of secreted CEACAM proteins by the mutation of the splice donor site of A domain exons in the microbat Myotis lucifugus. The splice donor site (consensus sequence is shown on top) is mutated (marked with the black box) leading to read-through into the intron and the generation of one or two in-frame stop codons (indicated in red and red boxes). Closely related A domain exons from whole genome shot gun sequences were aligned and a selection is depicted with indicated reading frame. The accession numbers are indicated in the left margin. The numbers to the left of the sequences indicate their positions within the shot gun sequence fragments.
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Kammerer and Zimmermann BMC Biology 2010 8:12 doi:10.1186/1741-7007-8-12