Figure 5.

The effect of CTCF30 on white variegation in In(1)wm4 and the fourth chromosome variegating strains 6-M193 and 39C-33. (a) Pigment levels were measured for In(1)wm4/w1118;CTCF30/+ and In(1)wm4/Y;CTCF30/+ genotypes compared with the corresponding sibling In(1)wm4;Tb/+ controls. In(1)wm4 heterozygous for CTCF30 results in an increase in white expression; however, this increase is only significant in female progeny (white bars). Red pigment quantification mean values for each group are based on n = 10 (50 heads total). Error bars represent standard deviation, and values significantly different from the controls are marked with an asterisk (P < 0.001). (b) Pigment levels were independently measured for both the maternally (6-M193MAT) and paternally (6-M193PAT) inherited fourth chromosome variegator 6-M193. 6-M193 heterozygous for CTCF30 results in an increase in white expression when either maternally or paternally inherited; however, this increase is only significant in female progeny (white bars). Red pigment quantification mean values for each group are based on n = 9 (45 heads total) for 6-M193MAT male and female progeny; n = 10 (50 heads total) for 6-M193PAT male and female progeny; 6-M193;+/+ control male progeny; and n = 12 (60 heads total) for 6-M193;+/+ control female progeny. Error bars represent standard deviation, and values significantly different from the controls are marked with an asterisk (P < 0.001). (c) Pigment levels were independently measured for both the maternally (39C-33MAT) and paternally (39C-33PAT) inherited fourth chromosome variegator 39C-33. 39C-33 heterozygous for CTCF30 results in no significant change in white expression when either maternally or paternally inherited. Red pigment quantification mean values for each group are based on n = 10 (50 heads total) for 39C-33MAT and 39C-33PAT male and female progeny, and n = 20 (100 heads total) for 39C-33;+/+ control male and female progeny. Error bars represent standard deviation.

MacDonald et al. BMC Biology 2010 8:105   doi:10.1186/1741-7007-8-105
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