Figure 3.

Absence of maternal or paternal effects from mutant dCTCF on Dp(1;f)LJ9 garnet expression. External control progeny (no modifier allele) have the same genotype as internal control progeny (y1zag53d/Dp(1;f)LJ9; TM3, Sb Ser/+) but are generated from a separate cross with parents that have never encountered a mutant CTCFX allele. (a) Maternally inherited Dp(1;f)LJ9 CTCFX internal control eye pigment levels; no significant difference in pigment levels was observed between external control progeny (no modifier allele) and internal control progeny from fathers carrying CTCFX (CTCFX internal), demonstrating that no paternal effect occurs. (b) Phenotypes of maternally inherited Dp(1;f)LJ9 ranging from 0% to 100% pigmentation; No modifier allele n = 300, CTCFEY15833 internal n = 300, CTCF30 internal n = 300. No garnet variegation was detected from the internal controls. (c) Paternally inherited Dp(1;f)LJ9 CTCF internal control eye pigment levels; no significant difference in pigment levels was observed between the external control progeny (no modifier allele) and internal control progeny from mothers carrying CTCFX (CTCFX internal), demonstrating that no maternal effect occurs. (d) Phenotypes of paternally inherited Dp(1;f)LJ9 ranging from 0% to 100% pigmentation; no modifier allele n = 300, CTCFEY15833 internal n = 300, CTCF30 internal n = 300. No significant change in garnet variegation was detected from the internal controls. Red eye pigment levels are measured by absorbance at 480 nm, and pigment quantification mean values for each group are based on n = 5 samples (40 heads total); error bars represent standard deviation.

MacDonald et al. BMC Biology 2010 8:105   doi:10.1186/1741-7007-8-105
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