Table 6 |
||||||||
|
Verification of microarray results by Real-time PCR. |
||||||||
|
Real time PCR IR vs OUT |
Affymetrix microarray |
|||||||
|
Gene |
Day |
IR* |
OUT* |
SED** |
Fold Change |
P-value |
Fold Change |
P-value |
|
|
||||||||
|
FKBP5 |
5 |
5.56 ± 0.76 |
4.77 ± 0.72 |
0.42 |
-1.73 |
0.092 |
-1.56 |
0.014 |
|
IRF7 |
5 |
6.12 ± 1.43 |
7.70 ± 1.04 |
0.72 |
2.99 |
0.056 |
5.00 |
0.003 |
|
IRP6 |
5 |
5.62 ± 2.97 |
8.75 ± 1.54 |
1.37 |
8.71 |
0.054 |
8.28 |
0.008 |
|
MT1J |
5 |
2.31 ± 3.61 |
7.78 ± 1.88 |
1.66 |
44.33 |
0.012 |
28.70 |
0.002 |
|
MX |
5 |
6.20 ± 2.35 |
8.08 ± 0.45 |
0.98 |
3.66 |
0.110 |
4.67 |
0.017 |
|
PDK4 |
5 |
9.30 ± 1.13 |
8.39 ± 0.38 |
0.49 |
-1.88 |
0.111 |
-3.19 |
0.005 |
|
SQLE |
5 |
5.85 ± 1.51 |
7.59 ± 1.44 |
0.85 |
3.34 |
0.069 |
5.69 |
0.044 |
|
CCL8 |
28 |
8.28 ± 0.87 |
10.29 ± 1.29 |
0.68 |
4.04 |
0.021 |
4.41 |
0.000 |
|
CXCL12 |
28 |
4.44 ± 0.23 |
5.15 ± 0.27 |
0.14 |
1.63 |
0.001 |
1.83 |
0.003 |
|
CXCL9 |
28 |
4.91 ± 1.30 |
7.37 ± 2.36 |
1.10 |
5.52 |
0.057 |
6.28 |
0.007 |
|
IRF7 |
28 |
5.21 ± 1.55 |
6.20 ± 0.70 |
0.69 |
1.99 |
0.196 |
3.56 |
0.006 |
|
MT1J |
28 |
3.33 ± 2.10 |
6.58 ± 1.58 |
1.07 |
9.49 |
0.014 |
13.01 |
0.001 |
|
PDK4 |
28 |
9.75 ± 0.66 |
7.61 ± 0.78 |
0.42 |
-4.43 |
0.000 |
-11.62 |
0.000 |
|
SQLE |
28 |
5.14 ± 1.12 |
7.27 ± 2.04 |
0.95 |
4.38 |
0.056 |
6.34 |
0.001 |
|
TFRC |
28 |
5.78 ± 1.38 |
7.62 ± 0.89 |
0.67 |
3.58 |
0.024 |
6.88 |
0.000 |
|
PDK4 |
56 |
8.73 ± 0.54 |
7.55 ± 0.42 |
0.28 |
-2.27 |
0.002 |
-3.82 |
0.007 |
|
SELL |
56 |
18.82 ± 0.88 |
16.10 ± 3.29 |
1.39 |
-6.60 |
0.101 |
-4.03 |
0.003 |
|
Real time PCR IN vs OUT |
Affymetrix microarray |
|||||||
|
Gene |
Day |
IN* |
OUT* |
SED** |
Fold Change |
P-value |
Fold Change |
P-value |
|
TFRC |
5 |
9.15 ± 0.64 |
7.78 ± 1.20 |
0.56 |
-2.58 |
0.041 |
-5.01 |
0.005 |
|
CXCR4 |
28 |
9.09 ± 1.01 |
8.17 ± 1.18 |
0.63 |
-1.89 |
0.177 |
3.91 |
0.010 |
|
CCL28 |
56 |
8.61 ± 0.74 |
9.75 ± 0.95 |
0.49 |
2.20 |
0.044 |
2.64 |
0.025 |
|
CCL8 |
56 |
9.19 ± 0.80 |
10.44 ± 1.31 |
0.63 |
2.38 |
0.080 |
2.44 |
0.009 |
|
PI3 |
56 |
5.67 ± 1.26 |
9.16 ± 1.28 |
0.82 |
11.17 |
0.004 |
6.80 |
0.002 |
|
IFIT2 |
56 |
5.63 ± 2.12 |
7.72 ± 1.79 |
1.13 |
4.26 |
0.095 |
8.32 |
0.009 |
|
PDK4 |
56 |
6.68 ± 0.25 |
7.55 ± 0.42 |
0.20 |
1.83 |
0.002 |
1.75 |
0.008 |
|
SQLE |
56 |
6.30 ± 0.92 |
7.40 ± 0.93 |
0.53 |
2.15 |
0.065 |
2.90 |
0.011 |
|
|
||||||||
|
Real-time PCR results at each time-point for the comparisons IR vs OUT and IN vs OUT. *PCR values are expressed as mean ΔCt ± SD, N = 6. **Standard error of difference |
||||||||
|
Mulder et al. BMC Biology 2009 7:79 doi:10.1186/1741-7007-7-79 |
||||||||
