Both basal polarity and collagen IV contribute to apical polarity in HTP cultures. A. Histogram of the percentages of acini formed by S1 cells (S1 acini) with lateroapical ZO-1 under different culture conditions (5% HTP, 10% HTP, control 5% 3D-Matrigel™ drip for eight and 10 days). B. Histograms of the percentages of S1 acini with lateroapical ZO-1, after treatment from day 5 to 8 with function blocking antibody for β1-integrin [β1-block] and β4-integrin [β4-block], and IgG in 5% HTP culture. C. Histograms of the percentages of S1 acini with lateroapical ZO-1 after culture in 2% HTP, 2% HTP + collagen IV (Col IV); 2% HTP + laminin 111 (lam 1); 2% HTP + collagen IV (Col IV) and laminin 111 (lam 1), during eight days of culture. *P < 0.05; **P < 0.01.
Plachot et al. BMC Biology 2009 7:77 doi:10.1186/1741-7007-7-77