Figure 5.

Both basal polarity and collagen IV contribute to apical polarity in HTP cultures. A. Histogram of the percentages of acini formed by S1 cells (S1 acini) with lateroapical ZO-1 under different culture conditions (5% HTP, 10% HTP, control 5% 3D-Matrigel™ drip for eight and 10 days). B. Histograms of the percentages of S1 acini with lateroapical ZO-1, after treatment from day 5 to 8 with function blocking antibody for β1-integrin [β1-block] and β4-integrin [β4-block], and IgG in 5% HTP culture. C. Histograms of the percentages of S1 acini with lateroapical ZO-1 after culture in 2% HTP, 2% HTP + collagen IV (Col IV); 2% HTP + laminin 111 (lam 1); 2% HTP + collagen IV (Col IV) and laminin 111 (lam 1), during eight days of culture. *P < 0.05; **P < 0.01.