Open Access Methodology article

Factors necessary to produce basoapical polarity in human glandular epithelium formed in conventional and high-throughput three-dimensional culture: example of the breast epithelium

Cedric Plachot1, Lesley S Chaboub1, Hibret A Adissu1, Lei Wang1, Albert Urazaev2, Jennifer Sturgis1, Elikplimi K Asem1 and Sophie A Lelièvre1*

Author Affiliations

1 Department of Basic Medical Sciences and Purdue Center for Cancer Research; Purdue University, West Lafayette, IN 47907, USA

2 Department of Biological Sciences; Purdue University, West Lafayette, IN 47907, USA

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BMC Biology 2009, 7:77  doi:10.1186/1741-7007-7-77

Published: 16 November 2009

Additional files

Additional file 1:

Additional information. This file includes, in order, additional methods, additional table S1 (Table S1), additional table S2 (Table S2), additional figure S1 (Figure S1), additional figure S2 (Figure S2), additional figure S3 (Figure S3), additional figure S4 (Figure S4), movie legends).

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Open Data

Additional file 2:

Movie 1. 3D reconstruction of the lumen in S1 cells in 3D-Matrigel drip culture.

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Additional file 3:

Movie 2. 3D reconstruction of an acinus in S1 cells in 3D-Matrigel drip culture

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Open Data

Additional file 4:

Movie 3. 3D reconstruction of an acinus formed by S1 cells in HTP culture.

Format: AVI Size: 181KB Download file

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Open Data