Additional file 3.
Movie S3 - Membrane localization of green fluorescent protein (GFP)-tagged FtsY(NG-1) mutant derivative. The GFP-tagged FtsY(NG+1) derivative was expressed from a plasmid in DH5α and localization was monitored on a motorised Zeiss Axioobserver microscope using a Cascade EM-CCD camera. The FtsY(NG+1) is an N-terminally truncated FtsY-derivative, which contains only one out of two lipid binding domains and displays reduced activity. Z stacks were acquired with 0.1 μm spacing and were three dimensionally de-convoluted using Autodeblur software. Movies were created in Metamorph and can be displayed with Quicktime movie player.
Format: MOV Size: 821KB Download file
Mircheva et al. BMC Biology 2009 7:76 doi:10.1186/1741-7007-7-76