Additional file 2.


Movie S2 - Membrane localization of green fluorescent protein (GFP)-tagged FtsY(B3) mutant derivative. The GFP-tagged FtsY(B3) mutant derivative was expressed from a plasmid in DH5α and localization was monitored on a motorised Zeiss Axioobserver microscope using a Cascade EM-CCD camera. The FtsY(B3) derivative exhibits reduced activity due to a triple-point mutation within the first lipid-binding domain of FtsY. Z stacks were acquired with 0.1 μm spacing and were three dimensionally de-convoluted using Autodeblur software. Movies were created in Metamorph and can be displayed with Quicktime movie player.

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Mircheva et al. BMC Biology 2009 7:76   doi:10.1186/1741-7007-7-76