Table 5

Test for lethality and sterility

First round of F₃ single-pair matings (SPM)

Second round of F₃ single-pair matings (SPM)

Offspring

Phenotype

Offspring

Phenotype

At least one SPM indicates homozygosity

Viable

At least one SPM (in total) indicates homozygosity

Viable

All informative* SPM indicate heterozygosity of both parents

Potentially lethal

All informative^* SPM indicate heterozygosity of both parents

Lethal

At least two SPM without any offspring but with living parents

Potentially sterile

Unable to find at least four SPM (in total) without any offspring but with living parents (method 1) OR unable to identify either a fertile homozygous female or a fertile homozygous male (method 2)

Sterile

After the first round of single-pair matings (SPM), all viable insertions were discarded (unless an enhancer trap was detected). All potentially lethal and potentially sterile lines were retested in a second round of single-pair matings.

*A single-pair mating is uninformative if it produces no offspring and one or both parents are dead, or less than 20 offspring are present and all of them are GFP-positive (see Table 4 and Methods)

Trauner et al. BMC Biology 2009 7:73   doi:10.1186/1741-7007-7-73