Table 4

Evaluation of F3 single-pair matings

Offspring of a single-pair mating

Interpretation/Result


No offspring, but parents alive at the time of evaluation

Indicates sterility of one or both parents

No offspring, but one or both parents dead at the time of evaluation

uninformative single-pair mating*

At least one EGFP-negative progeny

This indicates heterozygosity of both parents.

All progeny EGFP positive, at least 20 beetles present

This indicates homozygosity of one or both parents

All progeny EGFP positive, but less than 20 beetles present

uninformative single-pair mating*


*These single-pair matings were omitted from the overall evaluation (see Methods)

Trauner et al. BMC Biology 2009 7:73   doi:10.1186/1741-7007-7-73

Open Data