Table 3 |
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Primers used for reverse transcription polymerase chain reaction analysis and sequence confirmation. |
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|
Use |
Primer |
Oligonucleotide sequence |
|
|
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|
RT-PCR |
all1101-F |
CGCTGCTCTATCGCCTGACTG |
|
|
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|
all1101-R |
GCGTACTTTCCAGCGATTGTGC |
|
|
|
||
|
all2148-F |
ACGGTGACGGGGGAAACAGGA |
|
|
|
||
|
all2148-R |
ACTTCCACTCGCTCAATCGTCC |
|
|
|
||
|
all2158-F |
CACCACCAGCAGAACCAACAGC |
|
|
|
||
|
all2158-R |
CGATAAATCCACCTCACCACGG |
|
|
|
||
|
all2236-F |
AATCGCGCGGCACTCTACCGT |
|
|
|
||
|
all2236-R |
GGATAACTCCAATCCCACGAGC |
|
|
|
||
|
all2610-F |
CAGTCATAGGAGAGGCGGGATT |
|
|
|
||
|
all2610-R |
CATAGAGTACAGAAGCCGGTCC |
|
|
|
||
|
all2620-F |
AAACTCCCTCAACCGCGCTGG |
|
|
|
||
|
all2620-R |
GGAATCCGCGAACCATCGGC |
|
|
|
||
|
all2674-F |
CCCAGAAACACCTACCGCAGAA |
|
|
|
||
|
all2674-R |
AACTAGGTTGACGACCCCACCT |
|
|
|
||
|
all3310-F |
TTTAGGCAACCCAGGCGGCAC |
|
|
|
||
|
all3310-R |
GCATTATGCTCAAAGGTGACGCG |
|
|
|
||
|
all4026-F |
GTGGTTTTTGTGGAGTGTGGGG |
|
|
|
||
|
all4026-R |
CCATCAACTGGTGTGTCTTCCC |
|
|
|
||
|
all4924-F |
CCCTACCAGAGGATCTGGGGA |
|
|
|
||
|
all4924-R |
CTGTACCAACGGCTGGTAAGAC |
|
|
|
||
|
alr0397-F |
TGCGTCGCGGGATTTGCGAAC |
|
|
|
||
|
alr0397-R |
GGATAGTATTGACCCTGGGGTC |
|
|
|
||
|
alr2153-F |
CCTCCCGTGGGATTAACTTTGG |
|
|
|
||
|
alr2153-R |
CGCATCAGGGCCCACTCGA |
|
|
|
||
|
alr2175-F |
GGTGTCCCGGCTGTTGGTACT |
|
|
|
||
|
alr2175-R |
AGTACCTCAAACCTCTCTGGGC |
|
|
|
||
|
alr2185-F |
CCCCGTCAGGTACTCGAAGAC |
|
|
|
||
|
alr2185-R |
TCTTCCATGTCAAGCTAGGGGC |
|
|
|
||
|
alr2209-F |
CCTACTCCCACACCCCCAAC |
|
|
|
||
|
alr2209-R |
CTGTCTTGGTCAGGTCTTCGGG |
|
|
|
||
|
alr2211-F |
CAAGATCGCCAAGTGGTGAGGC |
|
|
|
||
|
alr2211-R |
GTGTTTCCAGGTAACAAGCCCC |
|
|
|
||
|
alr2581-F |
GCGGGGACAGAAGGCAAATTTG |
|
|
|
||
|
alr2581-R |
CGCCTAATTGTTCTGTACCCCC |
|
|
|
||
|
alr2588-F |
CAGGTGAGGCGGGATTACCTG |
|
|
|
||
|
alr2588-R |
ACTCCCCCTGGTTCTAGTTGTC |
|
|
|
||
|
alr2592-F |
CCCCAAAGCCAGTGGAGGGA |
|
|
|
||
|
alr2592-R |
CCCCTAGCGGCTCGAACATTG |
|
|
|
||
|
alr2596-F |
CGCCTGTGCGCGATATTCCTG |
|
|
|
||
|
alr2596-R |
CAGGCGCAACAAATACCCGTTC |
|
|
|
||
|
alr2626-F |
GGCGTTCAACCAGGGGGAGT |
|
|
|
||
|
alr2626-R |
CTAAACCAGGTCTGGGTATGGC |
|
|
|
||
|
alr3242-F |
CCCGACGTGATAGTGGGTCAC |
|
|
|
||
|
alr3242-R |
CTGGGGGAATCCGGCTGCAT |
|
|
|
||
|
rnpB-F |
AGGGAGAGAGTAGG GTTGG |
|
|
|
||
|
rnpB-R |
GGTTTACCGAGCCAGTACCTCT |
|
|
|
||
|
Sequence confirmation |
all2619-20 F |
CTCCCATTTCTCCGAAGCTG |
|
|
||
|
all2619-20 R |
CAACGCTGGGGCCAACATAG |
|
|
|
||
|
alr4028-29 F |
CTATGGACTTAACCAACAAAGCATTC |
|
|
|
||
|
alr4028-29 R |
CTTCTCTGGTTTAAGGTCAGGATTAC |
|
|
|
||
|
Mirus et al. BMC Biology 2009 7:68 doi:10.1186/1741-7007-7-68 |
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