Additional file 1.

Additional figure. Immunohistochemistry for c-Myc. (a) Fetal liver of a day 10.5 embryo as positive control [22] showing strong nuclear staining in dark violet (counterstain pink). Scale bar, 50 μm. (b) Wild type (WT) and mutant mammary glands at second pregnancy day 6.5, when c-Myc expression is highest. WT epithelium shows clear dark staining compared with mutant glands, shown in the upper panel in violet (with pink counterstain) and in the lower panel in red (no counterstain). Note that in the mutant gland (lower panel) some epithelial clusters retained c-Myc (insert b, red staining) while all other clusters are clearly Myc-deficient (insert a, no staining). Scale bars, 50 μm.

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Stoelzle et al. BMC Biology 2009 7:63   doi:10.1186/1741-7007-7-63