Delayed proliferative response of c-Myc mutant cells in second pregnancy. (a) Detection of recombined allele in mutant glands as described in Figure 1(c). np = non-pregnant, parous. (b) Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) on c-myc and β-actin as described in Figure 1(d). (c) Immunohistochemistry for Ki-67 (left) and cyclin D1 (right) on wild type (WT) and mutant glands taken at day 6.5 and 14.5 of a second pregnancy. Ki-67 stains all actively cycling cells (brown), resting cells in G0 are counterstained (blue). Scale bar, 100 μm. (d) Expression levels of N- and L-myc determined via semi-quantitative RT-PCR.
Stoelzle et al. BMC Biology 2009 7:63 doi:10.1186/1741-7007-7-63