Research article
c-Myc affects mRNA translation, cell proliferation and progenitor cell function in the mammary gland
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* Corresponding author: Nancy E Hynes nancy.hynes@fmi.ch
1 Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
2 Division of Stem Cells and Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany
3 HI-STEM (Heidelberg Institute for Stem Cell Technology and Experimental Medicine), Heidelberg, Germany
BMC Biology 2009, 7:63 doi:10.1186/1741-7007-7-63
Published: 28 September 2009Additional files
Additional file 1:
Additional figure. Immunohistochemistry for c-Myc. (a) Fetal liver of a day 10.5 embryo as positive control [22] showing strong nuclear staining in dark violet (counterstain pink). Scale bar, 50 μm. (b) Wild type (WT) and mutant mammary glands at second pregnancy day 6.5, when c-Myc expression is highest. WT epithelium shows clear dark staining compared with mutant glands, shown in the upper panel in violet (with pink counterstain) and in the lower panel in red (no counterstain). Note that in the mutant gland (lower panel) some epithelial clusters retained c-Myc (insert b, red staining) while all other clusters are clearly Myc-deficient (insert a, no staining). Scale bars, 50 μm.
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Additional file 2:
Supplementary methods. Condition for quantitative polymerase chain reaction (PCR) and all primers used in semi-quantitative and quantitative PCR analyses.
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