Additional file 5.
Paxillin is a downstream target of JNK signalling. Larval eye/antennal imaginal discs containing eyFLP-induced MARCM clones (green), with attached brain lobes (bl) in (E). Grey scale is Paxillin. (A) FRT82B scrib1. scrib mutant clones have elevated levels of Paxillin in some mutant cells (arrow). (B) FRT82B UAS-bskDN. Ectopic expression of BskDN in clones does not alter Paxillin levels in the eye disc. (C) FRT82B scrib1 UAS-bskDN. scrib mutant clones expressing BskDN no longer show elevated levels of Paxillin, although non-clonal tissue adjacent to mutant clones sometimes does ectopically express Paxillin (arrow), presumably reflecting JNK activation in the non-mutant tissue. (D) UAS-dRas1V12; FRT82B. Ectopic expression of RasACT in clones does not generally elevate Paxillin levels. (E) UAS-dRas1V12; FRT82B scrib1. Paxillin levels are increased in scrib1 + RasACT tumours, most notably at the invasive front, as shown by tumour cells invading into the brain lobes. (F) UAS-hepACT; FRT82B UAS-P35. Clones of tissue expressing an activated allele of the Drosophila JNKK homologue, hemipterous, Hep (HepACT), kept alive through the co-expression of the caspase inhibitor P35, ectopically express elevated levels of Paxillin.
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Leong et al. BMC Biology 2009 7:62 doi:10.1186/1741-7007-7-62