Additional file 5.

Paxillin is a downstream target of JNK signalling. Larval eye/antennal imaginal discs containing eyFLP-induced MARCM clones (green), with attached brain lobes (bl) in (E). Grey scale is Paxillin. (A) FRT82B scrib¹. scrib mutant clones have elevated levels of Paxillin in some mutant cells (arrow). (B) FRT82B UAS-bsk^DN. Ectopic expression of Bsk^DN in clones does not alter Paxillin levels in the eye disc. (C) FRT82B scrib¹ UAS-bsk^DN. scrib mutant clones expressing Bsk^DN no longer show elevated levels of Paxillin, although non-clonal tissue adjacent to mutant clones sometimes does ectopically express Paxillin (arrow), presumably reflecting JNK activation in the non-mutant tissue. (D) UAS-dRas1^V12; FRT82B. Ectopic expression of Ras^ACT in clones does not generally elevate Paxillin levels. (E) UAS-dRas1^V12; FRT82B scrib¹. Paxillin levels are increased in scrib¹ + Ras^ACT tumours, most notably at the invasive front, as shown by tumour cells invading into the brain lobes. (F) UAS-hep^ACT; FRT82B UAS-P35. Clones of tissue expressing an activated allele of the Drosophila JNKK homologue, hemipterous, Hep (Hep^ACT), kept alive through the co-expression of the caspase inhibitor P35, ectopically express elevated levels of Paxillin.

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Leong et al. BMC Biology 2009 7:62   doi:10.1186/1741-7007-7-62