Additional file 3.
Ectopic expression of activated aPKC disrupts cell morphology and results in ectopic cell proliferation. eyFLP-induced MARCM clones (green). Grey scale is Elav (A, B, E-G) and BrdU (C, D, H, I). Phalloidin marks F-actin in red (A, B, E-G). A white bar indicates the location of the MF. (A-D) FRT82B UAS-DaPKCΔN. Ectopic expression of aPKCΔN in clones results in reduced amounts of clonal tissue that is mostly excluded basally from the epithelium and does not express Elav (A, B) and does not noticeably over-proliferate (C-D), although any proliferative defects are likely to be masked by cell death. (E-I) FRT82B UAS-DaPKCΔN UAS-bskDN. The co-expression of BskDN with aPKCΔN rescues the small clone phenotype of aPKCΔN clones alone and most of the mutant tissue has aberrant cell morphology and is extruded basally to form large masses of undifferentiated tissue beneath the dorsal and ventral sides of the eye disc epithelium (E-G). The mutant cells ectopically proliferate posterior to the MF, but not within the MF, in both apical and basal sections (H, I).
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Leong et al. BMC Biology 2009 7:62 doi:10.1186/1741-7007-7-62