aPKCCAAXDN restrains scrib1+ NACT neoplastic overgrowth. Larval eye/antennal imaginal discs containing eyFLP-induced MARCM clones (green) at day 5 (A, B, E, G-H) and day 9 (C, D, F). Grey scale is Elav and Red is Phalloidin to mark F-actin. (A, C) UAS-Nintra FRT82B scrib1. Expression of NACT in scrib mutant clones results in large tumours at day 5 (A) and these become massive (compare to FRT82B control clones in E) and fuse with the brain lobes (bl) by day 9 (C). (B, D, F) UAS-Nintra; FRT82B scrib1 UAS-DaPKCCAAXDN. Co-expression of aPKCCAAXDN with NACT in scrib mutant clones fails to restore tumour differentiation but retards tumour overgrowth at day 5 (B compared to A), and this becomes more apparent by day 9 (D compared to C), although tumour cells are still observed between the brain lobes (arrows in F). (G) UAS-Nintra; FRT82B UAS-DaPKCCAAXDN. Co-expression aPKCCAAXDN with NACT does not abrogate NACT-driven overgrowth of the eye/antennal disc. (H) UAS-Nintra; FRT82B scrib1 UAS-bskDN UAS-DaPKCCAAXDN. Expression of aPKCCAAXDN and BskDN with NACT in scrib mutant clones prevents neoplastic tumor overgrowth and restores the characteristically overgrown mosaic discs of NACT-expressing clones.
Leong et al. BMC Biology 2009 7:62 doi:10.1186/1741-7007-7-62