BskDN blocks scrib1 + NACT neoplastic overgrowth but does not restore differentiation. Larval eye/antennal imaginal discs containing eyFLP-induced MARCM clones (green) at approximately day 7 (A) and day 5 (B-K). Eye discs remain attached to each brain lobe (bl) in A-C. Grey scale is Elav and Red is Phalloidin to mark F-actin. (A, D, E) UAS-Nintra; FRT82B scrib1. Expression of NACT in scrib mutant clones results in tumour overgrowth with cells appearing to migrate (arrow) between the brain lobes (Elav positive) at day 7 (A) and failing to differentiate in apical and basal sections of the eye disc (D, E). (B, C, F-H) UAS-Nintra; FRT82B scrib1 UAS-bskDN. Co-expression of BskDN with NACT in scrib mutant clones results in larvae pupating at day5/6, thus precluding analysis of invasion at day 7. However, at day 5 no invasion is seen to occur between the brain lobes (B, C), despite tumour cells remaining undifferentiated (F, G) and forming large benign overgrowths (H). (I) UAS-Nintra; FRT82B. Expression of NACT alone in clones results in massively overgrown eye antennal discs. (J) UAS-Nintra; FRT82B UAS-bskDN. Co-expression of NACT with BskDN also results in massively overgrown eye/antennal discs. (K) FRT82B UAS-bskDN. BskDN-expressing eye/antennal discs are of normal size and differentiation.
Leong et al. BMC Biology 2009 7:62 doi:10.1186/1741-7007-7-62