Figure 6.

UAS-CG6234IR induction in D. melanogaster embryos. (A and B) Whole-mount immunohistochemical images of embryos (dorsal views) stained with anti-Hnt. (A) A wild-type embryo (stage 12) during germ band retraction. (B) An embryo expressing UAS-CG6234IR induced by nanos-GAL4. Hnt-positive nuclei of amnioserosa cells are indicated by black arrows. (C and D) Immunofluorescence confocal images of embryos (lateral views) stained with anti-Hnt (green) and To-PRO3 (blue). (C) Wild-type (stage 13) showing the amnioserosa nuclei that cover the dorsal region as a result of germ band retraction (white arrow). (D) UAS-CG6234IR ubiquitously expressed under nanos-GAL4 control produces embryos having disrupted germ band retraction (white arrows) and head defects (asterisks).